〔Abstract〕 Objective To analyze the value of the application of chemiluminescence immunoassay technology in the biochemical immunoassay of patients with thyroid tumors. Methods 106 cases of suspected thyroid tumor patients who were treated in Changsha Traditional Chinese Medicine Hospital from June 2017 to April 2019 were taken as observation objects. The patients were tested by radioimmunoassay technology and chemiluminescence immunoassay technology respectively, The pathological examination results were used as the gold standard. The detection specificity, sensitivity and accuracy of two methods are compared, and the patient's thyroglobulin level is determined. Results Among 106 patients with suspected thyroid tumors, 100 were diagnosed by pathological examination, accounting for 94.34%. The diagnostic accuracy, specificity, and sensitivity of chemiluminescence immunoassay technology were higher than those of radioimmunoassay technology, and the differences were statistically significant. (P < 0.05), the level of thyroglobulin detected by chemiluminescence immunoassay technology was significantly higher than that by radioimmunoassay technology, and the difference was statistically significant (P < 0.05). Conclusion Chemiluminescence immunoassay technology is more accurate in the diagnosis of thyroid tumors, and can provide a more effective reference for clinical and treatment.