〔Abstract〕 Objective To explore the effects of personalized breast milk fortifiers on the growth and development of premature infants and the incidence of complications. Methods A total of 86 preterm infants who were transferred to Wuxi Children’s Hospital of Nanjing Medical University within 24 hours after birth between April 2020 and April 2021 were selected. The preterm infants were divided into control groups according to different feeding Methods (standard breast milk fortification). Remedy feeding and observation group (individualized feeding with breast milk fortifier), 43 cases in each. Observe the growth and development of premature infants before and after feeding, serum biochemical indicators and the incidence of related complications during hospitalization. Results After feeding, the weight, length and head circumference of premature infants in the observation group were higher than those of the control group, and the difference was statistically significant (P < 0.05); the levels of PA, ALP, ALB and BUN of premature infants in the observation group after feeding were higher than those in the control group. In the control group, the difference was statistically significant (P < 0.05); there was no statistically significant difference in the incidence of complications between the two groups of preterm infants during hospitalization (P > 0.05). Conclusion The personalized addition of breast milk fortifiers can promote the growth and development of premature infants, and improve the nutritional level of premature infants. It has high safety and can ensure the healthy growth of children.