〔Abstract〕 Objective To investigate the effect of Taxus chinensis polysaccharide on proliferation and apoptosis of cervical cancer HeLa cell line and its mechanism. Methods Human cervical cancer HeLa cells in logarithmic growth stage were cultured in vitro and treated with different concentrations of Taxus chinensis polysaccharide (30, 60, 90, 120 μmol·L-1) were divided into 4 groups, and the control group was added with the same amount of 0.9% sodium chloride solution. MTT assay was used to detect cell proliferation, flow cytometry was used to detect cell apoptosis, and Western blot was used to detect the expression of survivin, Caspase-3, Bcl-2 and p53 proteins. Results Compared with the control group, 30 μmol·L-1 group, 60 μmol·L-1 group, 90 μmol·L-1 group, 120 μmol·L-1 of the proliferation inhibition rate and apoptosis rate of HeLa cells were significantly increased (P < 0.05). And the expression of p53 protein ncreased significantly, and the protein surface levels of Survivin, Bcl-2 and Caspase-3decreased significantly (P < 0.05). Conclusion Taxus chinensis polysaccharide can inhibit the proliferation and promote apoptosis of cervical cancer HeLa cells. Its mechanism may be related to up regulating the expression of Survivin, Bcl-2 and Caspase-3 and down regulating the expression of p53.