〔Abstract〕 Objective To analyze the clinical effect of insulin lispro in gestational diabetes mellitus. Methods 56 cases of gestational diabetes mellitus admitted to Huaiji People's Hospital from March 2018 to November 2019 were selected and divided into control group and observation group by random number table, with 28 cases in each group. The control group was treated with ordinary insulin and the observation group was treated with insulin lispro. The pregnancy outcome, the improvement of blood glucose level, the incidence of hypoglycemia and the acceptance of patients were compared between the two groups. Results There was no significant difference in the incidence of adverse pregnancy outcomes such as stillbirth, macrosomia, fetal distress, preterm delivery, gestational hypertension and ketosis between the two groups (P > 0.05). Before treatment, there was no significant difference between the two groups in the levels of fasting blood glucose (FBG), 2h postprandial blood glucose (2h PG) and glycosylated hemoglobin (HbA1c) (P > 0.05); After treatment, the levels of FBG, 2h PG and HbA1c in the two groups were lower than those before treatment, but there was no significant difference between the two groups (P > 0.05). The incidence of hypoglycemia in the observation group was significantly lower than that in the control group, and the acceptance rate was significantly higher than that in the control group (P < 0.05). Conclusion Insulin lispro dependent treatment in gestational diabetes mellitus has no significant difference in pregnancy outcome and glycemic control, but can reduce the incidence of hypoglycemia and improve the treatment of patients with gestational diabetes mellitus.