〔Abstract〕 Objective To study the influence of procalcitonin (PCT) and high-sensitivity C-reactive protein (hs-CRP) indicators in the early diagnosis of neonatal sepsis. Method A total of 60 newborns diagnosed with sepsis admitted to the Zhecheng County People’s Hospital from May 2017 to November 2019 were selected as the observation group, and 60 healthy newborns during the same period were selected as the control group to compare the differences between the two groups. The changes of PCT and hs-CRP indexes in the observation group before and after treatment. Results The observation group had more children with positive PCT, hs-CRP, WBC, and ESR indicators than the control group, and the difference was statistically significant (P < 0.05); the observation group had higher levels of PCT, hs-CRP, WBC, and ESR Compared with the control group, the difference was statistically significant (P < 0.05); compared with 1 day after treatment, the observation group had significantly lower PCT levels on 2 d, 4 d, and 6 d after treatment, and 4 d and 6 d after treatment hs-CRP levels were significantly reduced, and the difference was statistically significant (P < 0.05). Conclusion For neonates with sepsis, determining the PCT and hs-CRP indicators as early as possible in the diagnosis can be used as an important reference indicator and has a high reference value.