〔Abstract〕 Objective Analyze the effects of different temperatures and different storage times on the stability of plasma liverfunction biochemical indicators. Method From January 2020 to June 2021, 200 health examiners from Fengshun County People’s Hospital were taken as the research objects, and they were randomly divided into two groups, temperature group (storage at different temperatures in the group) and storage time group (storage time in the group) 100 cases in each group, and the test Results of the two groups were recorded. Results In the storage time group, 4 indexes of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and direct bilirubin (DBIL) were tested immediately and 7 days after storage There was no significant difference in the test results (P > 0.05); there was no significant difference in the test results of the four indicators of ALT, AST, TBIL and DBIL in the temperature group at room temperature and at 4 °C (P > 0.05). Conclusion Different temperature and storage time have little effect on the stability of plasma liver function and biochemical function indexes such as ALT, AST, TBIL and DBIL.