〔Abstract〕 Objective To study the correlation between male sperm DNA integrity, abnormal sperm rate and the frequency of spontaneous abortion, so as to evaluate whether sperm DNA fragment index (DFI) can be used as an independent predictor of spontaneous abortion. Methods the clinical data of 60 pregnant women with spontaneous abortion in reproductive medicine center of North Guangdong People's Hospital from January 2020 to January 2021 were analyzed retrospectively. According to the male sperm DNA fragmentation index (DFI), they were divided into observation group (DFI > 30%, 30 cases) and control group (DFI ≤ 30%, 30 cases). The correlation between age, abnormal sperm rate and the frequency of spontaneous abortion was analyzed. Results The SMR of men in the observation group was higher than that in the control group, and the age and the number of spontaneous abortions of their spouses were higher than those in the control group (P < 0.05); The number of spontaneous abortions in women was positively correlated with the age, SMR and DFI of their spouses (male) (r = 0.531, 0.742, 0.673, all P < 0.05). Conclusion Whether male sperm DNA is complete and whether sperm morphology is normal is closely related to the frequency of spontaneous abortion. DFI can be used as an independent predictor of spontaneous abortion.