〔Abstract〕 Objective To study the significance of arm venipuncture model in the teaching practice of blood donation. Methods The blood collection nurses who participated in the pre-job training in the blood station from June 2014 to August 2019 were selected as the research objects. According to whether the arm veinpuncture model was used for blood collection training, they were divided into the control group and the observation group. The actual operation assessment results of the two groups after 4 weeks of training were compared, and the satisfaction of blood collection nurses with the training model and blood donors for blood collection technology were investigated. Results The evaluation scores of the training nurses in the observation group were 100.00% (10/10), and the control group was 61.54%. The evaluation scores of the training nurses in the observation group were significantly higher than those of the control group, and the difference was statistically significant. (P < 0.05). The dissatisfaction rate of blood donors in the observation group was 0.024%, which was lower than 0.063% in the control group. The difference was statistically significant (P < 0.05). There was no statistically significant difference between the observation group and the control group in terms of training time and theoretical knowledge training satisfaction (P > 0.05). The observation group’s satisfaction with practical training was higher than that of the control group (P < 0.05). Conclusion The arm venipuncture model is scientific, advanced and close to human arm. It can be applied in the teaching of venous blood collection for blood donation to improve the teaching quality, to reduce the pain of blood donors due to unskilled puncture from the new blood collection nurses, and to improve the satisfaction of blood donors. It can be popularized in the teaching and training of blood centers.