〔Abstract〕 ObjectiveTo investigate the effects of PI3K/AKT signaling pathway inhibitor on the invasion and migration of esophageal cancer cell TE1, and to discusse the possible mechanism. Methods TE1 cells were cultured and treated with LY294002 (30 μmol/L) for 1 hour. The inhibition rate of cell proliferation was measured by MTT 24 hours later. Cell migration and invasion were observed by cell scratch test and cell invasion test. The expression level of PY14 Cav–1 was detected by Western blot. Results LY294002 could signifi cantly inhibit the growth of TE1 cell and inhibit its migration and invasion ability. There was a signifi cant difference between LY294002 group and control group (P < 0.05). At the same time, LY294002 could down-regulate the expression of PY14 Cav–1. Conclusion PI3K/AKT signaling pathway positively regulates the proliferation of esophageal cancer cells and participates in cells invasion and migration. LY294002 can inhibit the growth, migration and invasion of esophageal cancer cells. Its possible mechanism is related to down-regulation of PY14Cav–1 expression.