〔Abstract〕 Objective To investigate the association of dietary and other lifestyle behaviors with carotid artery plaque formation among Shenzhen’s healthy population. Methods This cross-sectional study was performed on 1861 participants who underwent physical examination in Shenzhen People’s Hospital from January 2019 to June 2019. Details of demographics, biochemical test, the results of ultrasonography and lifestyle questionnaire were collected for all the participants. Subjects were categorized as “carotid plaque” group (n = 427) or “non-carotid plaque” group (n = 1434) according to their carotid artery ultrasonography. Multivariate logistic regression models were used to analysis the factors of diet and lifestyle related to carotid artery plaque. Results The overall prevalence of carotid plaque was 22.9 % in this study. The mean age in the ”carotid plaque” group was much higher than those in the ”non-carotid plaque” group. With the growth of age, the incidence of carotid artery plaque increases and the detection rate of carotid plaque in males was signifi cantly higher than that in females. Age, rates of male, smoking, inadequate intake of fruit (< 100g per day), history of hypertension, history of diabetes and fatty liver in the “carotid plaque” group were significantly higher than those in the ”non-carotid plaque” group. Multivariable logistic regression analyses revealed that age, male, high glycated hemoglobin, fatty liver, history of hypertension, smoking and alcohol consumption were independently associated with the formation of carotid artery plaque in healthy people. Conclusion The present study shows that increasing age is independent risk factor of carotid plaque in healthy individuals. We recommend that carotid ultrasonography should be performed in people with fatty liver and history of hypertension, especially in smokers or men. However,lower hemoglobin a1C level, smoking cessation and moderate drinking may help us to prevent the formation and development of atherosclerosis.