〔Abstract〕 Objective To investigate the positive expression of wnt5a molecules after radiation intervention of C6 glioma stem cells (C6 GSCs). Methods BrdU incorporation experiment to clarify the cell proliferation; confocal microscopy to observe the cell location of wnt5a; protein electrophoresis to determine the level of wnt5a protein expression. Results Under the effectof conditioned medium, the number of C6 GSCs reached more than 98%; after radiation intervention, the 0.3 Gy group promoted the proliferation and differentiation of C6 GSCs; the 3.0 Gy group decreased the proliferation and differentiation; wnt5a was found in the nucleus, cytoplasm and There are expressions on the protrusions, and the difference between the 0.3 Gy group and the Con group is statistically signifi cant (P < 0.05); the gray-scale statistical analysis of protein electrophoresis is consistent with the above results. Conclusion A certain range of low-dose ionizing radiation stimulation can promote the proliferation and differentiation of C6 GSCs, and wnt5a molecules are positively expressed on C6 GSCs, suggesting that wnt molecules may be involved in mediating the stimulation effects of C6 GSCs ionizing radiation.