〔Abstract〕 Objective To analyze the value and the reason of misdiagnosis and missed diagnosis of congenital genital malformation by two-dimension and three-dimension ultrasound. Methods The ultrasound features of 48 patients who were diagnosed with congenital genital malformation by hysteroscope, laparoscope and uterine probe in the first affiliated hospital of Shenzhen university from February 2018 to September 2020 were selected. The segmental analysis method (including the uterine and the uterine cavity shape, the number of cervix and vagina) was used for retrospective analysis to analyze the reason of misdiagnosis and missed diagnosis. Results 48 cases were diagnosed with uterine malformation by ultrasound. 47 of them were correctly diagnosed by hysteroscope, laparoscope and uterine probe and 1 of them which was normal uterus was misdiagnosed incomplete mediastinal uterus. 8 cases were diagnosed with abnormal number of cervix (cervical canals), 1 case was missed, and 4 cases were misdiagnosed by ultrasound. 1 case was diagnosed with vaginal malformation (double vagina) and 4 cases with oblique vaginal septum were missed diagnosed by ultrasound. Conclusion The quality and classifi cation of congenital genital malformation might be improved by using segmental analysis method to integral assessment.